- Publisher
CABI - Published
30th November 2022 - ISBN 9781800621152
- Language English
- Pages 560 pp.
- Size 6" x 9"
Technology for modifying the genotypes and phenotypes of insects and other arthropods has steadily progressed by development of more precise and powerful methods, most prominently transgenic modification. There is now almost unlimited ability to modify phenotypes to benefit human health and agriculture. Precise DNA modifications and gene drive particularly have the power to make wild-type populations less harmful in ways that could never be performed with previous transgenic approaches. This transition from primarily laboratory science to greater prominence for field applications has also necessitated a greater development of modelling, ethical considerations and regulatory oversight.
The second edition of Transgenic Insects contains chapters contributed by experts in the field that cover the technology and applications that are now possible. These include an increased emphasis on acceptance issues that will be necessary for application of many technologies.
Chapter 1: Transposon-based Technologies for Insects
Chapter 2: Inducible and Repressible Systems for Transgene Expression
Chapter 3: Sex-, Tissue- and Stage-Specific Transgene Expression
Chapter 4: RNA Interference to Modify Phenotypes in Agriculturally Important Pest and Beneficial Insects: Useful Examples and Future Challenges
Chapter 5: Site-specific Recombination for Gene Locus-directed Transgene Integration and Modification
Chapter 6: Receptor-Mediated Ovary Transduction of Cargo - ReMOT Control: A Comprehensive Review and Detailed Protocol for Implementation
Chapter 7: Site-directed DNA Sequence Modification Using CRISPR-Cas9
Chapter 8: An Introduction to the Molecular Genetics of Gene Drives and Thoughts on Their Gradual Transition to Field Use
Chapter 9: Drosophila melanogaster As a Model for Gene Drive Systems
Chapter 10: Sex Ratio Manipulation Using Gene Drive for Mosquito Population Control
Chapter 11: Population Modification Using Gene Drive for Reduction of Malaria Transmission
Chapter 12: Modelling threshold-dependent gene drives
Chapter 13: Tsetse Paratransgenesis: a Novel Strategy for Reducing the Spread of African Trypanosomiases
Chapter 14: Paratransgenic Control of Chagas Disease
Chapter 15: Asaia Paratrangenesis in Mosquitoes
Chapter 16: Paratransgenesis in Mosquitoes and Other Insects: Microbial Ecology and Bacterial Genetic Considerations
Chapter 17: Transgenic approaches for sterile insect control of dipteran livestock pests and lepidopteran crop pests
Chapter 18: Honey bee genome editing
Chapter 19: Progress Towards Germline Transformation of Ticks
Chapter 20: Silkworm Transgenesis and its Applications
Chapter 21: Tephritid Fruit Fly Transgenesis and Applications
Chapter 22: Antiviral Effectors for Mosquito Transgenesis
Chapter 23: Self-Limiting Insects for Pest Management
Chapter 24: Public Acceptability and Stakeholder Engagement for Genetic Control Technologies
Chapter 25: Regulation of Transgenic Insects
Chapter 26: Economics of Transgenic Insects for Field Release
Chapter 27: The Cartagena Protocol on Biosafety and the Regulation of Transboundary Movement of Living Modified Organisms
Chapter 28: Risk Analysis of Transgenic Insects
Mark Quentin Benedict
Mark Quentin Benedict has a PhD in entomology with an emphasis on molecular biology and genetics from the University of Florida. He has developed technology for the development of transgenic insects; developed insectary methods for producing mosquitoes for release into the field; directed field studies underlying releases of transgenic insects; and assisted developing country operations where transgenic insects will be used. He has worked at USDA, the Centers for Disease Control and Prevention (CDC), the International Atomic Energy Agency and the University of Perugia. He is currently a research biologist at the CDC and is based in Atlanta, GA. He has contributed book chapters and over 100 peer reviewed publications.
Maxwell J. Scott
Maxwell J. Scott has a PhD in molecular cell biology with an emphasis on the regulation of gene expression from Baylor College of Medicine in Houston, Texas. He has developed techniques for insect transgenesis, engineered male-only strains of agricultural pests such as the New World screwworm and spotted wing Drosophila, led the assembly and analysis of genomes from several species including screwworm, participated in the application for field testing of a transgenic male-only screwworm strain and developed self-limiting gene drive technologies that are applicable to a broad range of insect pests. Max has worked at the University of North Carolina at Chapel Hill, the Institute of Biotechnology and Molecular Biology in Heraklion, Crete, Greece, Emory University, and was a lecturer in genetics for 16 years at Massey University in New Zealand. He is currently a Professor in the Entomology and Plant Pathology Department at North Carolina State University. He has contributed reviews, book chapters, a patent and peer reviewed publications.